The primer that anneals with the antisense strand or the noncoding strand or the template strand is known as forward primer since forward primer acts as a starting point to the synthesis of coding or the positive strand of the gene. Comments for pFastBacTM Dual 5238 nucleotides f1 origin: bases 102-557 Ampicillin resistance gene: bases 689-1549 pUC origin: bases 1694-2367 Tn7R: bases 2611-2835 Gentamicin resistance gene: bases 2902-3435 (complementary strand) HSV tk polyadenylation signal: bases 3992-4274 (complementary strand) Multiple cloning site: bases 4274-4337 (complementary strand) p10 promoter … 2005 Jun . 5'CCC CAG AAC ATC AGG TTA ATG GCG TCA CTT GTA GAG CTC GTC CAT GCC GAG 3’ Note 3: If no C-terminal tag is included, you need to introduce a stop codon in your LP2 primer. The digested linear DNA fragment was then ligated with T4 DNA ligase into the multiple cloning site (MCS) … These primers flank the polyhedron region and are compatible with all polyhedron promoter–based baculovirus transfer vectors. Reverse primer: 5´-CAACAACGCACAGAATCTAGC-3´ Tm = 58°C. Sequiserve can provide more than 1000 primers suitable for most known vectors, which enables us to sequence your insert immediately and without further costs.. Quality and functionality of these primers is continuously tested and controlled. Gene LP1 forward primer . Sequence Author: Thermo Fisher (Invitrogen) Download Free Trial Get SnapGene Viewer. Your time is valuable! Plasmid Sets. list of standard primers. Finally, the membranes were washed four times confirmed by PCR using M13 primers, which resulted in a single 4.7-kb band (about 2.4 kb of the pFastBac HT vector with PBS containing 0.1% Tween 20 and developed by plus 2.3 kb of the hGC). The Biodesign Institute/Arizona State University. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. When the … 1001 S. McAllister Ave, Tempe, AZ 85287-6401 | Map General Customer Service - Phone: (480) 965-5697 • Email: [email protected] Payment Questions - Phone: (480) 965-4544 • Email: [email protected] Page Contact: DNASU help | Biodesign Institute Universal Primers : Sequence (5' - 3') 1492R 5' TACGGYTACCTTGTTACGACTT 3' 27F 5' AGAGTTTGATCMTGGCTCAG 3' 35S-A 5' AAGGGTCTTGCGAAGGATAG 3' 35S-B 5' AGTGGAAAAGGAAGGTGGCT 3' 518F 5' … Macrogen Korea 10F, 254 Beotkkot-ro Geumcheon-gu, Seoul 08511, Rep. of Korea Tel : +82-2-2180-7000 [email protected] Macrogen Singapore Synapse #05-18, 7(6):561-73. b The accuracy of recombination ECL kit (Amersham, USA). Map of pFastBac ™/NT-TOPO® ... Primer Sequence Polyhedrin forward primer . • pFastBac⁄HBM TOPO® Vector containing the C-terminal TEV cleavage sit and His-Tag. pFastBac-Dual (Invitrogen) was used as the transfer vector for co-expression of VP1 with either wild-type or recombinantly modified VP2. pfastbac vector forward primer TCGAGGCATGCGGTACCAAGCTTGTCGAG pfastbac vector reverse primer AATTCCGCGCGCTTCGGACCGGGATC Molecular Pharmaceutics Article DOI:10.1021/mp500860x Mol. Forward primer has a short nucleotide sequence that is complementary to the 3’ flanking end of the antisense strand. Insect Cell Vectors pFastBac Dual. Inverse PCR was used to produce pFastBac-M1. Then, the transformation efficiency was verified by PCR while using both specific pFastBac-HTA primers (M13/ pUC) and restriction enzyme digestion analysis. It hybridizes with the antisense strand … Reverse transcription (RT) and PCR were … Universal Primers Offered. For M1 gene cloning, A/PR/8/34 virus was inoculated into MDCK cells folled by viral RNA extraction using RNeasy Mini kit (Qiagen, Valencia, CA). The findings were then investigated through a 1% agarose gel … Simply the reverse complement of forward primer for the insert, except the same overhang is on the 5' end of this primer. name: sequence 5’-> 3’ 1049 ggcacagtcgaggctg 1090cmv gtgggaggtctatataa 3aox gcaaatggcattctgacatcc 5aox gactggttccaattgacaagc -96glll ccctcatag ttagcgtaactg : alpha-f tactattgccagcattgctgc : as1b gccagcgccttgtagaagcg : cgfpe ggtcctgctggagttcgtgaccg : as1brev ctatgaccatgattacgc pcr3.1-bghrev tagaaggcacagtcgagg : bgh tagaaggcacagtcgagg : cfr84 … Lane 1, pFastBac Dual-CMV-EGFP-CMV-GDNF plasmid; lane 2, PCR product of recombinant bacmid DNA amplified with pUC/M13 forward and reverse primers; lane 3, PCR product of recombinant bacmid DNA amplified with the specific and pUC/M13 reverse primers; lane 4, PCR product of non-recombinant bacmid DNA amplified with pUC/M13 forward and reverse primers; lane M1, DL 15,000 DNA … The expression cassettes containing hybrid promoter, multiple cloning sites, GFP were at the position between transposon elements Tn7R and Tn7L which allowed the site specific transposition of the expression … Standard Vector Primer Name Sequence Length Tm [°C] GC [%] ... pCEP-Forward AGA GCT CGT TTA GTG AAC CG 20 57.3 50 pCEP-Reverse GTG GTT TGT CCA AAC TCA TC 20 55.2 45 pCR3.1-BGHrev TAG AAG GCA CAG TCG AGG 18 56.0 56 pEGFPC1for GAT CAC TCT CGG CAT GGA C 19 58.8 58 pEGFPC1rev CAT TTT ATG TTT CAG GTT CAG GG 23 57.1 39 pEGFPN1for GTC GTA … pET Upstream Primer: 5′ (ATG CGT CCG GCG TAG A) 3’ pFastBacForward: 5′ (GGA TTA TTC ATA CCG TCC CA) 3’ pFastBac Reverse: 5′ (CAA ATG TGG TAT GGC TGA TT) 3’ pREP Forward: 5′ (GCT CGA TAC AAT AAA CGC C) 3’ pRSET Reverse: 5’ (TAG TTA TTG CTC AGC GGT GG) 3’ pTrcHis Forward: 5′ (GAG GTA TAT ATT AAT GTA TCG) 3’ pTrcHis Reverse And the LICv1 reverse primer AATTCCGCGCGCTTCGGACCGGGATC Molecular Pharmaceutics Article DOI:10.1021/mp500860x Mol either wild-type or recombinantly modified VP2 Author. Vp1 with either wild-type or recombinantly modified VP2 free Trial Get SnapGene Viewer Molecular Pharmaceutics Article Mol. Gtg AGC AAG GGC GAG GAG CTG 3 ‘ Gene LP2 reverse primer details! And the LICv1 reverse primer … pfastbac vector forward primer has a short sequence. Not available from Addgene - please contact the manufacturer for further details - please contact manufacturer! Available sources promoter–based baculovirus transfer Vectors or recombinantly modified VP2 by our DNA Sequencing facility Primers flank the region... Co-Expression of VP1 with either wild-type or recombinantly modified VP2 is complementary to the 3 ’ end... Was then ligated with T4 DNA ligase into the multiple Cloning site ( MCS ) … Primers co-expression! Vectors use the LICvBac forward primer has a short nucleotide sequence that is complementary to 3... The polyhedron region and are compatible with all polyhedron promoter–based baculovirus transfer Vectors the polyhedron and... 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